A, For all assays, the chromosomal location of the gene is indicated (boxes), with the direction of transcription indicated by the arrow. The 5’ and 3’ BACs are indicated in ovals, with the number identifying each respective BAC and the color indicating the probe color in the accompanying images.
B, Representative FISH images of the various TMPRSS2-ETS aberrations observed in this study. Green and red arrows show individual signals, and yellow arrows indicate colocalized signals in 4’6-diamidino-2-phenylindole (DAPI) stained nuclei. B1, Colocalized signals in metastatic prostate cancer cells in a case lacking TMPRSS2 rearrangement. B2, TMPRSS2 rearrangement positive case without Edel, as indicated by split 5’ and 3’ signals. B3, TMPRSS2 rearrangement positive case with Edel, as indicated by one pair of co-localized signals (yellow arrow) and the loss of one red labeled probe 3’ to TMPRSS2. B4, Co-localized signals (yellow arrows) in a representative case lacking ERG rearrangement. B5, An ERG rearrangement positive case with Edel showing loss of one red labeled probe 5’ to ERG. B6, ETV1 rearrangement positive case without deletion, as indicated by split 5’ and 3’ signals (green and red arrows). B7, ETV1 rearrangement positive case with deletion exhibiting loss of one red labeled probe 5’ to ETV1. B8, ETV1 amplification shown by multiple (–) copies of co-localized signals in case 30 B9, A case showing rearrangement of ETV4, as indicated by break apart probes. Numbered BACs are as follows: 1= RP11-35C4 (5’ to TMPRSS2) and 2= RP11-120C17 (3’ to 18 TMPRSS2), 3= RP11-95I21 (5’ to ERG) and 4= RP11-476D17(3’ to ERG), 5= RP11- 661L15 (5’ to ETV1) and 6= RP11-124L22(3’ to ETV1) and 7= RP11-100E5 (5’ to ETV4) and 8= RP11-436J4 (3’ to ETV4).